Florida Biology EOC Practice Test

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Prepare for the Florida Biology EOC Test. Use our comprehensive flashcards and multiple-choice questions, complete with hints and explanations, to get ready for your Biology End-of-Course exam!

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During which stage of mitosis do sister chromatids line up in the middle of the cell?

Anaphase
Interphase
Metaphase
Prophase

The correct answer is: Anaphase

Sister chromatids line up in the middle of the cell during metaphase. This stage is characterized by the alignment of chromosomes along the equatorial plane of the cell, known as the metaphase plate. The spindle fibers attached to the kinetochores of the chromatids help ensure that each sister chromatid will be pulled towards opposite poles of the cell during the following stage, anaphase. In contrast, during anaphase, the sister chromatids are separated and begin moving to opposite sides of the cell. Interphase is the preparatory phase that occurs before mitosis and does not involve the organization of chromatids. Prophase is the stage where chromatin condenses into visible chromosomes, and the nuclear envelope begins to break down, but the chromatids have not yet aligned in the middle of the cell.